1KXK

Crystal Structure of a RNA Molecule Containing Domain 5 and 6 of the Yeast ai5g Group II Self-splicing Intron

1KXK の概要

• エントリーDOI: 10.2210/pdb1kxk/pdb
• 分子名称: ai5g group II Self-splicing intron, MAGNESIUM ION (2 entities in total)
• 機能のキーワード: double helix, tetraloop, tetraloop receptor, two-nucleotide bulge, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22669.09

構造登録者

Zhang, L.,Doudna, J.A. (登録日: 2002-01-31, 公開日: 2002-03-22, 最終更新日: 2024-02-14)

主引用文献

Zhang, L.,Doudna, J.A.
Structural insights into group II intron catalysis and branch-site selection.
Science, 295:2084-2088, 2002

PubMed Abstract: Group II self-splicing introns catalyze autoexcision from precursor RNA transcripts by a mechanism strikingly similar to that of the spliceosome, an RNA-protein assembly responsible for splicing together the protein-coding parts of most eukaryotic pre-mRNAs. Splicing in both cases initiates via nucleophilic attack at the 5' splice site by the 2' OH of a conserved intron adenosine residue, creating a branched (lariat) intermediate. Here, we describe the crystal structure at 3.0 A resolution of a 70-nucleotide RNA containing the catalytically essential domains 5 and 6 of the yeast ai5gamma group II self-splicing intron, revealing an unexpected two-nucleotide bulged structure around the branch-point adenosine in domain 6.

PubMed: 11859154
DOI: 10.1126/science.1069268

実験手法

X-RAY DIFFRACTION (3 Å)