1KTQ

DNA POLYMERASE

1KTQ の概要

• エントリーDOI: 10.2210/pdb1ktq/pdb
• 分子名称: DNA POLYMERASE I (2 entities in total)
• 機能のキーワード: nucleotidyltransferase, dna-replication
• 由来する生物種: Thermus aquaticus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 61250.34

構造登録者

Korolev, S.,Waksman, G. (登録日: 1995-08-16, 公開日: 1996-11-08, 最終更新日: 2024-02-14)

主引用文献

Korolev, S.,Nayal, M.,Barnes, W.M.,Di Cera, E.,Waksman, G.
Crystal structure of the large fragment of Thermus aquaticus DNA polymerase I at 2.5-A resolution: structural basis for thermostability.
Proc.Natl.Acad.Sci.USA, 92:9264-9268, 1995

PubMed Abstract: The crystal structure of the large fragment of the Thermus aquaticus DNA polymerase (Klentaq1), determined at 2.5-A resolution, demonstrates a compact two-domain architecture. The C-terminal domain is identical in fold to the equivalent region of the Klenow fragment of Escherichia coli DNA polymerase I (Klenow pol I). Although the N-terminal domain of Klentaq1 differs greatly in sequence from its counterpart in Klenow pol I, it has clearly evolved from a common ancestor. The structure of Klentaq1 reveals the strategy utilized by this protein to maintain activity at high temperatures and provides the structural basis for future improvements of the enzyme.

PubMed: 7568114
DOI: 10.1073/pnas.92.20.9264

実験手法

X-RAY DIFFRACTION (2.5 Å)