1KOA

TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS

Summary for 1KOA

• Entry DOI: 10.2210/pdb1koa/pdb
• Descriptor: TWITCHIN (1 entity in total)
• Functional Keywords: kinase, twitchin, intrasteric regulation
• Biological source: Caenorhabditis elegans
• Total number of polymer chains: 1
• Total formula weight: 56426.31

Authors

Kobe, B.,Heierhorst, J.,Feil, S.C.,Parker, M.W.,Benian, G.M.,Weiss, K.R.,Kemp, B.E. (deposition date: 1996-06-28, release date: 1997-03-12, Last modification date: 2024-02-14)

Primary citation

Kobe, B.,Heierhorst, J.,Feil, S.C.,Parker, M.W.,Benian, G.M.,Weiss, K.R.,Kemp, B.E.
Giant protein kinases: domain interactions and structural basis of autoregulation.
EMBO J., 15:6810-6821, 1996

PubMed Abstract: The myosin-associated giant protein kinases twitchin and titin are composed predominantly of fibronectin- and immunoglobulin-like modules. We report the crystal structures of two autoinhibited twitchin kinase fragments, one from Aplysia and a larger fragment from Caenorhabditis elegans containing an additional C-terminal immunoglobulin-like domain. The structure of the longer fragment shows that the immunoglobulin domain contacts the protein kinase domain on the opposite side from the catalytic cleft, laterally exposing potential myosin binding residues. Together, the structures reveal the cooperative interactions between the autoregulatory region and the residues from the catalytic domain involved in protein substrate binding, ATP binding, catalysis and the activation loop, and explain the differences between the observed autoinhibitory mechanism and the one found in the structure of calmodulin-dependent kinase I.

PubMed: 9003756

Experimental method

X-RAY DIFFRACTION (3.3 Å)