1KOA

TWITCHIN KINASE FRAGMENT (C.ELEGANS), AUTOREGULATED PROTEIN KINASE AND IMMUNOGLOBULIN DOMAINS

1KOA の概要

• エントリーDOI: 10.2210/pdb1koa/pdb
• 分子名称: TWITCHIN (1 entity in total)
• 機能のキーワード: kinase, twitchin, intrasteric regulation
• 由来する生物種: Caenorhabditis elegans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 56426.31

構造登録者

Kobe, B.,Heierhorst, J.,Feil, S.C.,Parker, M.W.,Benian, G.M.,Weiss, K.R.,Kemp, B.E. (登録日: 1996-06-28, 公開日: 1997-03-12, 最終更新日: 2024-02-14)

主引用文献

Kobe, B.,Heierhorst, J.,Feil, S.C.,Parker, M.W.,Benian, G.M.,Weiss, K.R.,Kemp, B.E.
Giant protein kinases: domain interactions and structural basis of autoregulation.
EMBO J., 15:6810-6821, 1996

PubMed Abstract: The myosin-associated giant protein kinases twitchin and titin are composed predominantly of fibronectin- and immunoglobulin-like modules. We report the crystal structures of two autoinhibited twitchin kinase fragments, one from Aplysia and a larger fragment from Caenorhabditis elegans containing an additional C-terminal immunoglobulin-like domain. The structure of the longer fragment shows that the immunoglobulin domain contacts the protein kinase domain on the opposite side from the catalytic cleft, laterally exposing potential myosin binding residues. Together, the structures reveal the cooperative interactions between the autoregulatory region and the residues from the catalytic domain involved in protein substrate binding, ATP binding, catalysis and the activation loop, and explain the differences between the observed autoinhibitory mechanism and the one found in the structure of calmodulin-dependent kinase I.

PubMed: 9003756

実験手法

X-RAY DIFFRACTION (3.3 Å)