1KKH

Crystal Structure of the Methanococcus jannaschii Mevalonate Kinase

Summary for 1KKH

• Entry DOI: 10.2210/pdb1kkh/pdb
• Descriptor: Mevalonate Kinase, 1,4-DIETHYLENE DIOXIDE (3 entities in total)
• Functional Keywords: mixed beta sheet, phosphate-binding loop, beta-alpha-beta, transferase
• Biological source: Methanocaldococcus jannaschii
• Cellular location: Cytoplasm : Q58487
• Total number of polymer chains: 1
• Total formula weight: 35941.80

Authors

Yang, D.,Shipman, L.W.,Roessner, C.A.,Scott, A.I.,Sacchettini, J.C. (deposition date: 2001-12-08, release date: 2002-03-27, Last modification date: 2024-10-30)

Primary citation

Yang, D.,Shipman, L.W.,Roessner, C.A.,Scott, A.I.,Sacchettini, J.C.
Structure of the Methanococcus jannaschii mevalonate kinase, a member of the GHMP kinase superfamily.
J.Biol.Chem., 277:9462-9467, 2002

PubMed Abstract: The mevalonate-dependent pathway is used by many organisms to synthesize isopentenyl pyrophosphate, the building block for the biosynthesis of many biologically important compounds, including farnesyl pyrophosphate, dolichol, and many sterols. Mevalonate kinase (MVK) catalyzes a critical phosphoryl transfer step, producing mevalonate 5'-phosphate. The crystal structure of thermostable MVK from Methanococcus jannaschii has been determined at 2.4 A, revealing an overall fold similar to the homoserine kinase from M. jannaschii. In addition, the enzyme shows structural similarity with mevalonate 5-diphosphate decarboxylase and domain IV of elongation factor G. The active site of MVK is in the cleft between its N- and C-terminal domains. Several structural motifs conserved among species, including a phosphate-binding loop, have been found in this cavity. Asp(155), an invariant residue among MVK sequences, is located close to the putative phosphate-binding site and has been assumed to play the catalytic role. Analysis of the MVK model in the context of the other members of the GHMP kinase family offers the opportunity to understand both the mechanism of these enzymes and the structural details that may lead to the design of novel drugs.

PubMed: 11751891
DOI: 10.1074/jbc.M110787200

Experimental method

X-RAY DIFFRACTION (2.4 Å)