1KIL

Three-dimensional structure of the complexin/SNARE complex

Summary for 1KIL

• Entry DOI: 10.2210/pdb1kil/pdb
• Descriptor: Synaptobrevin SNARE motif, Syntaxin SNARE motif short, SNAP-25 N-terminal SNARE motif, ... (7 entities in total)
• Functional Keywords: helix bound to four helix bundle, membrane protein
• Biological source: Rattus norvegicus (Norway rat); More
• Cellular location: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P63045; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein (By similarity): P32851; Cytoplasm, perinuclear region (By similarity): P60880 P60880; Cytoplasm, cytosol: P63041
• Total number of polymer chains: 5
• Total formula weight: 36923.73

Authors

Chen, X.,Tomchick, D.,Kovrigin, E.,Arac, D.,Machius, M.,Sudhof, T.C.,Rizo, J. (deposition date: 2001-12-03, release date: 2002-03-13, Last modification date: 2023-08-16)

Primary citation

Chen, X.,Tomchick, D.R.,Kovrigin, E.,Arac, D.,Machius, M.,Sudhof, T.C.,Rizo, J.
Three-dimensional structure of the complexin/SNARE complex.
Neuron, 33:397-409, 2002

PubMed Abstract: During neurotransmitter release, the neuronal SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 form a four-helix bundle, the SNARE complex, that pulls the synaptic vesicle and plasma membranes together possibly causing membrane fusion. Complexin binds tightly to the SNARE complex and is essential for efficient Ca(2+)-evoked neurotransmitter release. A combined X-ray and TROSY-based NMR study now reveals the atomic structure of the complexin/SNARE complex. Complexin binds in an antiparallel alpha-helical conformation to the groove between the synaptobrevin and syntaxin helices. This interaction stabilizes the interface between these two helices, which bears the repulsive forces between the apposed membranes. These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release.

PubMed: 11832227
DOI: 10.1016/S0896-6273(02)00583-4

Experimental method

X-RAY DIFFRACTION (2.3 Å)