1KIL

Three-dimensional structure of the complexin/SNARE complex

1KIL の概要

• エントリーDOI: 10.2210/pdb1kil/pdb
• 分子名称: Synaptobrevin SNARE motif, Syntaxin SNARE motif short, SNAP-25 N-terminal SNARE motif, ... (7 entities in total)
• 機能のキーワード: helix bound to four helix bundle, membrane protein
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein: P63045; Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type IV membrane protein (By similarity): P32851; Cytoplasm, perinuclear region (By similarity): P60880 P60880; Cytoplasm, cytosol: P63041
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 36923.73

構造登録者

Chen, X.,Tomchick, D.,Kovrigin, E.,Arac, D.,Machius, M.,Sudhof, T.C.,Rizo, J. (登録日: 2001-12-03, 公開日: 2002-03-13, 最終更新日: 2023-08-16)

主引用文献

Chen, X.,Tomchick, D.R.,Kovrigin, E.,Arac, D.,Machius, M.,Sudhof, T.C.,Rizo, J.
Three-dimensional structure of the complexin/SNARE complex.
Neuron, 33:397-409, 2002

PubMed Abstract: During neurotransmitter release, the neuronal SNARE proteins synaptobrevin/VAMP, syntaxin, and SNAP-25 form a four-helix bundle, the SNARE complex, that pulls the synaptic vesicle and plasma membranes together possibly causing membrane fusion. Complexin binds tightly to the SNARE complex and is essential for efficient Ca(2+)-evoked neurotransmitter release. A combined X-ray and TROSY-based NMR study now reveals the atomic structure of the complexin/SNARE complex. Complexin binds in an antiparallel alpha-helical conformation to the groove between the synaptobrevin and syntaxin helices. This interaction stabilizes the interface between these two helices, which bears the repulsive forces between the apposed membranes. These results suggest that complexin stabilizes the fully assembled SNARE complex as a key step that enables the exquisitely high speed of Ca(2+)-evoked neurotransmitter release.

PubMed: 11832227
DOI: 10.1016/S0896-6273(02)00583-4

実験手法

X-RAY DIFFRACTION (2.3 Å)