1KH6
Crystal Structure of an RNA Tertiary Domain Essential to HCV IRES-mediated Translation Initiation.
Summary for 1KH6
| Entry DOI | 10.2210/pdb1kh6/pdb |
| Descriptor | JIIIabc (1 entity in total) |
| Functional Keywords | translation, rna structure, ires, hcv, bromine, four-way junction, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 17634.66 |
| Authors | Kieft, J.S.,Zhou, K.,Grech, A.,Jubin, R.,Doudna, J.A. (deposition date: 2001-11-29, release date: 2002-04-26, Last modification date: 2024-02-14) |
| Primary citation | Kieft, J.S.,Zhou, K.,Grech, A.,Jubin, R.,Doudna, J.A. Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation. Nat.Struct.Biol., 9:370-374, 2002 Cited by PubMed Abstract: The hepatitis C virus (HCV) internal ribosome entry site (IRES) RNA drives internal initiation of viral protein synthesis during host cell infection. In the tertiary structure of the IRES RNA, two helical junctions create recognition sites for direct binding of the 40S ribosomal subunit and eukaryotic initiation factor 3 (eIF3). The 2.8 A resolution structure of the IIIabc four-way junction, which is critical for binding eIF3, reveals how junction nucleotides interact with an adjacent helix to position regions directly involved in eIF3 recognition. Two of the emergent helices stack to form a nearly continuous A-form duplex, while stacking of the other two helices is interrupted by the insertion of junction residues into the helix minor groove. This distorted stack probably serves as an important recognition surface for the translational machinery. PubMed: 11927953DOI: 10.1038/nsb781 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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