1KH6

Crystal Structure of an RNA Tertiary Domain Essential to HCV IRES-mediated Translation Initiation.

1KH6 の概要

• エントリーDOI: 10.2210/pdb1kh6/pdb
• 分子名称: JIIIabc (1 entity in total)
• 機能のキーワード: translation, rna structure, ires, hcv, bromine, four-way junction, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17634.66

構造登録者

Kieft, J.S.,Zhou, K.,Grech, A.,Jubin, R.,Doudna, J.A. (登録日: 2001-11-29, 公開日: 2002-04-26, 最終更新日: 2024-02-14)

主引用文献

Kieft, J.S.,Zhou, K.,Grech, A.,Jubin, R.,Doudna, J.A.
Crystal structure of an RNA tertiary domain essential to HCV IRES-mediated translation initiation.
Nat.Struct.Biol., 9:370-374, 2002

PubMed Abstract: The hepatitis C virus (HCV) internal ribosome entry site (IRES) RNA drives internal initiation of viral protein synthesis during host cell infection. In the tertiary structure of the IRES RNA, two helical junctions create recognition sites for direct binding of the 40S ribosomal subunit and eukaryotic initiation factor 3 (eIF3). The 2.8 A resolution structure of the IIIabc four-way junction, which is critical for binding eIF3, reveals how junction nucleotides interact with an adjacent helix to position regions directly involved in eIF3 recognition. Two of the emergent helices stack to form a nearly continuous A-form duplex, while stacking of the other two helices is interrupted by the insertion of junction residues into the helix minor groove. This distorted stack probably serves as an important recognition surface for the translational machinery.

PubMed: 11927953
DOI: 10.1038/nsb781

実験手法

X-RAY DIFFRACTION (2.9 Å)