1KGZ

Crystal Structure Analysis of the Anthranilate Phosphoribosyltransferase from Erwinia carotovora (current name, Pectobacterium carotovorum)

Summary for 1KGZ

• Entry DOI: 10.2210/pdb1kgz/pdb
• Related: 1KHD
• Descriptor: Anthranilate phosphoribosyltransferase, MERCURY (II) ION, MANGANESE (II) ION, ... (5 entities in total)
• Functional Keywords: type 3 prt fold, nucleotide binding fold, transferase
• Biological source: Pectobacterium carotovorum
• Total number of polymer chains: 2
• Total formula weight: 75515.31

Authors

Kim, C.,Xuong, N.-H.,Edwards, S.,Madhusudan,Yee, M.-C.,Spraggon, G.,Mills, S.E. (deposition date: 2001-11-28, release date: 2002-10-23, Last modification date: 2024-02-14)

Primary citation

Kim, C.,Xuong, N.-H.,Edwards, S.,Madhusudan,Yee, M.-C.,Spraggon, G.,Mills, S.E.
The Crystal Structure of Anthranilate Phosphoribosyltransferase from the Enterobacterium Pectobacterium carotovorum
FEBS Lett., 523:239-246, 2002

PubMed Abstract: The structure of anthranilate phosphoribosyltransferase from the enterobacterium Pectobacterium carotovorum has been solved at 2.4 A in complex with Mn(2+)-pyrophosphate, and at 1.9 A without ligands. The enzyme structure has a novel phosphoribosyltransferase (PRT) fold and displays close homology to the structures of pyrimidine nucleoside phosphorylases. The enzyme is a homodimer with a monomer of 345 residues. Each monomer consists of two subdomains, alpha and alpha/beta, which form a cleft containing the active site. The nature of the active site is inferred from the trapped MnPPi complex and detailed knowledge of the active sites of nucleoside phosphorylases. With the anthranilate (An)PRT structure solved, the structures of all the enzymes required for tryptophan biosynthesis are now known.

PubMed: 12123839
DOI: 10.1016/S0014-5793(02)02905-8

Experimental method

X-RAY DIFFRACTION (2.4 Å)