1KFC

CRYSTAL STRUCTURE OF ALPHAT183V MUTANT OF TRYPTOPHAN SYNTHASE FROM SALMONELLA TYPHIMURIUM With Indole Propanol Phosphate

1KFC の概要

• エントリーDOI: 10.2210/pdb1kfc/pdb
• 関連するPDBエントリー: 1k8x
• 分子名称: TRYPTOPHAN SYNTHASE ALPHA CHAIN, TRYPTOPHAN SYNTHASE BETA CHAIN, INDOLE-3-PROPANOL PHOSPHATE, ... (6 entities in total)
• 機能のキーワード: helix, lyase
• 由来する生物種: Salmonella typhimurium; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 72141.04

構造登録者

Kulik, V.,Weyand, M.,Siedel, R.,Niks, D.,Arac, D.,Dunn, M.F.,Schlichting, I. (登録日: 2001-11-20, 公開日: 2003-01-07, 最終更新日: 2023-08-16)

主引用文献

Kulik, V.,Weyand, M.,Siedel, R.,Niks, D.,Arac, D.,Dunn, M.F.,Schlichting, I.
On the Role of AlphaTHR183 in the Allosteric Regulation and Catalytic Mechanism of Tryptophan Synthase
J.Mol.Biol., 324:677-690, 2002

PubMed Abstract: The catalytic activity and substrate channeling of the pyridoxal 5'-phosphate-dependent tryptophan synthase alpha(2)beta(2) complex is regulated by allosteric interactions that modulate the switching of the enzyme between open, low activity and closed, high activity states during the catalytic cycle. The highly conserved alphaThr183 residue is part of loop alphaL6 and is located next to the alpha-active site and forms part of the alpha-beta subunit interface. The role of the interactions of alphaThr183 in alpha-site catalysis and allosteric regulation was investigated by analyzing the kinetics and crystal structures of the isosteric mutant alphaThr183Val. The mutant displays strongly impaired allosteric alpha-beta communication, and the catalytic activity of the alpha-reaction is reduced one hundred fold, whereas the beta-activity is not affected. The structural work establishes that the basis for the missing inter-subunit signaling is the lack of loop alphaL6 closure even in the presence of the alpha-subunit ligands, 3-indolyl-D-glycerol 3'-phosphate, or 3-indolylpropanol 3'-phosphate. The structural basis for the reduced alpha-activity has its origins in the missing hydrogen bond between alphaThr183 and the catalytic residue, alphaAsp60.

PubMed: 12460570
DOI: 10.1016/S0022-2836(02)01109-9

実験手法

X-RAY DIFFRACTION (1.5 Å)