1KF4

Atomic Resolution Structure of RNase A at pH 6.3

1KF4 の概要

• エントリーDOI: 10.2210/pdb1kf4/pdb
• 関連するPDBエントリー: 1KF2 1KF3 1KF5 1KF7 1KF8
• 分子名称: pancreatic ribonuclease, SULFATE ION (3 entities in total)
• 機能のキーワード: rnase a, titration, ph, crystal, soaking, hydrolase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13804.39

構造登録者

Berisio, R.,Sica, F.,Lamzin, V.S.,Wilson, K.S.,Zagari, A.,Mazzarella, L. (登録日: 2001-11-19, 公開日: 2001-12-19, 最終更新日: 2024-11-20)

主引用文献

Berisio, R.,Sica, F.,Lamzin, V.S.,Wilson, K.S.,Zagari, A.,Mazzarella, L.
Atomic resolution structures of ribonuclease A at six pH values.
Acta Crystallogr.,Sect.D, 58:441-450, 2002

PubMed Abstract: The diffraction pattern of protein crystals extending to atomic resolution guarantees a very accurate picture of the molecular structure and enables the study of subtle phenomena related to protein functionality. Six structures of bovine pancreatic ribonuclease at the pH* values 5.2, 5.9, 6.3, 7.1, 8.0 and 8.8 and at resolution limits in the range 1.05-1.15A have been refined. An overall description of the six structures and several aspects, mainly regarding pH-triggered conformational changes, are described here. Since subtle variations were expected, a thorough validation assessment of the six refined models was first carried out. Some stereochemical parameters, such as the N[bond]C(alpha)[bond]C angle and the pyramidalization at the carbonyl C atoms, indicate that the standard target values and their weights typically used in refinement may need revision. A detailed comparison of the six structures has provided experimental evidence on the role of Lys41 in catalysis. Furthermore, insights are given into the structural effects related to the pH-dependent binding of a sulfate anion, which mimics the phosphate group of RNA, in the active site. Finally, the results support a number of thermodynamic and kinetic experimental data concerning the role of the disulfide bridge between Cys65 and Cys72 in the folding of RNase A.

PubMed: 11856829
DOI: 10.1107/S0907444901021758

実験手法

X-RAY DIFFRACTION (1.1 Å)