1KAM

Structure of Bacillus subtilis Nicotinic Acid Mononucleotide Adenylyl Transferase

Summary for 1KAM

• Entry DOI: 10.2210/pdb1kam/pdb
• Related: 1KAQ
• Descriptor: NICOTINATE-NUCLEOTIDE ADENYLYLTRANSFERASE (2 entities in total)
• Functional Keywords: rossmann fold, transferase
• Biological source: Bacillus subtilis
• Total number of polymer chains: 4
• Total formula weight: 90475.61

Authors

Olland, A.M.,Underwood, K.W.,Czerwinski, R.M.,Lo, M.C.,Aulabaugh, A.,Bard, J.,Stahl, M.L.,Somers, W.S.,Sullivan, F.X.,Chopra, R. (deposition date: 2001-11-02, release date: 2002-07-12, Last modification date: 2024-02-07)

Primary citation

Olland, A.M.,Underwood, K.W.,Czerwinski, R.M.,Lo, M.C.,Aulabaugh, A.,Bard, J.,Stahl, M.L.,Somers, W.S.,Sullivan, F.X.,Chopra, R.
Identification, characterization, and crystal structure of Bacillus subtilis nicotinic acid mononucleotide adenylyltransferase.
J.Biol.Chem., 277:3698-3707, 2002

PubMed Abstract: The nadD gene, encoding the enzyme nicotinic acid mononucleotide (NaMN) adenylyltransferase (AT), is essential for the synthesis of NAD and subsequent viability of the cell. The nadD gene in Bacillus subtilis (yqeJ) was identified by sequence homology with other bacterial nadD genes and by biochemical characterization of the gene product. NaMN AT catalyzes the reversible adenylation of both NaMN and the nicotinamide mononucleotide (NMN) but shows specificity for the nicotinate. In contrast to other known NMN ATs, biophysical characterizations reveal it to be a dimer. The NaMN AT crystal structure was determined for both the apo enzyme and product-bound form, to 2.1 and 3.2 A, respectively. The structures reveal a "functional" dimer conserved in both crystal forms and a monomer fold common to members of the nucleotidyl-transferase alpha/beta phosphodiesterase superfamily. A structural comparison with family members suggests a new conserved motif (SXXXX(R/K)) at the N terminus of an alpha-helix, which is not part of the shared fold. Interactions of the nicotinic acid with backbone atoms indicate the structural basis for specificity.

PubMed: 11704676
DOI: 10.1074/jbc.M109670200

Experimental method

X-RAY DIFFRACTION (2.1 Å)