1KA9

Imidazole Glycerol Phosphate Synthase

1KA9 の概要

• エントリーDOI: 10.2210/pdb1ka9/pdb
• 分子名称: imidazole glycerol phosphate synthase (3 entities in total)
• 機能のキーワード: synthase, riken structural genomics/proteomics initiative, rsgi, structural genomics, transferase
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48836.07

構造登録者

Omi, R.,Miyahara, I.,Hirotsu, K.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2001-11-01, 公開日: 2002-12-04, 最終更新日: 2024-03-13)

主引用文献

Omi, R.,Mizuguchi, H.,Goto, M.,Miyahara, I.,Hayashi, H.,Kagamiyama, H.,Hirotsu, K.
Structure of imidazole glycerol phosphate synthase from Thermus thermophilus HB8: open-closed conformational change and ammonia tunneling.
J.Biochem., 132:759-765, 2002

PubMed Abstract: Imidazole glycerol phosphate synthase (IGPs) catalyzes the fifth step in the histidine biosynthetic pathway located at the branch point to de novo purine biosynthesis. IGPs is a multienzyme comprising glutaminase and synthase subunits. The glutaminase activity, which hydrolyzes glutamine to give ammonia, is coupled with substrate binding to the synthase subunit. The three-dimensional structure of the IGPs from Thermus thermophilus HB8 has been determined at 2.3 A resolution, and compared with the previously determined structures for the yeast and Thermotoga maritima enzymes. The structure of each subunit is similar to that of the corresponding domain in the yeast enzyme or subunit in the T. maritima enzyme. However, the overall structure is significantly different from the yeast and T. maritima enzymes, indicating that IGPs may change the relative orientation between the two subunits and close the glutaminase site upon glutamine binding. The putative ammonia tunnel, which carries nascent ammonia from glutaminase to the synthase site, has a closed gate comprising a cyclic salt bridge formed by four charged residues of the synthase subunit. The side chain of Lys100 in the cyclic salt bridge might change its side chain direction to form new interactions with the main chain carbonyl group of glutamine from the synthase subunit and the hydoxyl group of tyrosine from the glutaminase subunit, resulting in the opening of the gate for ammonia transfer.

PubMed: 12417026
DOI: 10.1093/oxfordjournals.jbchem.a003284

実験手法

X-RAY DIFFRACTION (2.3 Å)