1K8W

Crystal structure of the E. coli pseudouridine synthase TruB bound to a T stem-loop RNA

1K8W の概要

• エントリーDOI: 10.2210/pdb1k8w/pdb
• 分子名称: 5'-R(*GP*GP*CP*AP*AP*CP*GP*GP*UP*(FHU)P*CP*GP*AP*UP*CP*CP*CP*GP*UP*UP*GP*C)-3', tRNA Pseudouridine Synthase B, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: protein-rna complex, t stem-loop, trna, lyase-rna complex, lyase/rna
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 44661.39

構造登録者

Hoang, C.,Ferre-D'Amare, A.R. (登録日: 2001-10-25, 公開日: 2001-12-31, 最終更新日: 2024-02-07)

主引用文献

Hoang, C.,Ferre-D'Amare, A.R.
Cocrystal structure of a tRNA Psi55 pseudouridine synthase: nucleotide flipping by an RNA-modifying enzyme.
Cell(Cambridge,Mass.), 107:929-939, 2001

PubMed Abstract: Pseudouridine (Psi) synthases catalyze the isomerization of specific uridines in cellular RNAs to pseudouridines and may function as RNA chaperones. TruB is responsible for the Psi residue present in the T loops of virtually all tRNAs. The close homolog Cbf5/dyskerin is the catalytic subunit of box H/ACA snoRNPs. These carry out the pseudouridylation of eukaryotic rRNA and snRNAs. The 1.85 A resolution structure of TruB bound to RNA reveals that this enzyme recognizes the preformed three-dimensional structure of the T loop, primarily through shape complementarity. It accesses its substrate uridyl residue by flipping out the nucleotide and disrupts the tertiary structure of tRNA. Structural comparisons with TruB demonstrate that all Psi synthases are descended from a common molecular ancestor.

PubMed: 11779468
DOI: 10.1016/S0092-8674(01)00618-3

実験手法

X-RAY DIFFRACTION (1.85 Å)