1K8P

Structure of the Human G-quadruplex reveals a novel topology

1K8P の概要

• エントリーDOI: 10.2210/pdb1k8p/pdb
• 分子名称: 5'-D(*(BRU)P*AP*GP*GP*GP*(BRU)P*TP*AP*GP*GP*GP*T)-3', POTASSIUM ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: quadruplex dna, double chain reversal loop, dimeric quadruplex, human telomere sequence, g(anti).g(anti).g(anti).g(anti), parallel stranded, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7907.59

構造登録者

Parkinson, G.N.,Lee, M.P.H.,Neidle, S. (登録日: 2001-10-25, 公開日: 2002-05-31, 最終更新日: 2024-02-07)

主引用文献

Parkinson, G.N.,Lee, M.P.,Neidle, S.
Crystal structure of parallel quadruplexes from human telomeric DNA.
Nature, 417:876-880, 2002

PubMed Abstract: Telomeric ends of chromosomes, which comprise noncoding repeat sequences of guanine-rich DNA, are fundamental in protecting the cell from recombination and degradation. Disruption of telomere maintenance leads to eventual cell death, which can be exploited for therapeutic intervention in cancer. Telomeric DNA sequences can form four-stranded (quadruplex) structures, which may be involved in the structure of telomere ends. Here we describe the crystal structure of a quadruplex formed from four consecutive human telomeric DNA repeats and grown at a K(+) concentration that approximates its intracellular concentration. K(+) ions are observed in the structure. The folding and appearance of the DNA in this intramolecular quadruplex is fundamentally different from the published Na(+)-containing quadruplex structures. All four DNA strands are parallel, with the three linking trinucleotide loops positioned on the exterior of the quadruplex core, in a propeller-like arrangement. The adenine in each TTA linking trinucleotide loop is swung back so that it intercalates between the two thymines. This DNA structure suggests a straightforward path for telomere folding and unfolding, as well as ways in which it can recognize telomere-associated proteins.

PubMed: 12050675
DOI: 10.1038/nature755

実験手法

X-RAY DIFFRACTION (2.4 Å)