1K6X

Crystal structure of Nmra, a negative transcriptional regulator in complex with NAD at 1.5 A resolution (Trigonal form)

1K6X の概要

• エントリーDOI: 10.2210/pdb1k6x/pdb
• 関連するPDBエントリー: 1K6I 1K6J
• 分子名称: NmrA, CHLORIDE ION, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (4 entities in total)
• 機能のキーワード: rossmann fold transcriptional regulation short chain dehydrogenase reductase nadh binding, transcription
• 由来する生物種: Emericella nidulans
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39711.38

構造登録者

Stammers, D.K.,Ren, J.,Leslie, K.,Nichols, C.E.,Lamb, H.K.,Cocklin, S.,Dodds, A.,Hawkins, A.R. (登録日: 2001-10-17, 公開日: 2002-02-20, 最終更新日: 2023-08-16)

主引用文献

Stammers, D.K.,Ren, J.,Leslie, K.,Nichols, C.E.,Lamb, H.K.,Cocklin, S.,Dodds, A.,Hawkins, A.R.
The structure of the negative transcriptional regulator NmrA reveals a structural superfamily which includes the short-chain dehydrogenase/reductases.
EMBO J., 20:6619-6626, 2002

PubMed Abstract: NmrA is a negative transcriptional regulator involved in the post-translational modulation of the GATA-type transcription factor AreA, forming part of a system controlling nitrogen metabolite repression in various fungi. X-ray structures of two NmrA crystal forms, both to 1.8 A resolution, show NmrA consists of two domains, including a Rossmann fold. NmrA shows an unexpected similarity to the short-chain dehydrogenase/reductase (SDR) family, with the closest relationship to UDP-galactose 4-epimerase. We show that NAD binds to NmrA, a previously unreported nucleotide binding property for this protein. NmrA is unlikely to be an active dehydrogenase, however, as the conserved catalytic tyrosine in SDRs is absent in NmrA, and thus the nucleotide binding to NmrA could have a regulatory function. Our results suggest that other transcription factors possess the SDR fold with functions including RNA binding. The SDR fold appears to have been adapted for other roles including non-enzymatic control functions such as transcriptional regulation and is likely to be more widespread than previously recognized.

PubMed: 11726498
DOI: 10.1093/emboj/20.23.6619

実験手法

X-RAY DIFFRACTION (1.5 Å)