1K6D

CRYSTAL STRUCTURE OF ACETATE COA-TRANSFERASE ALPHA SUBUNIT

1K6D の概要

• エントリーDOI: 10.2210/pdb1k6d/pdb
• 分子名称: ACETATE COA-TRANSFERASE ALPHA SUBUNIT, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: structural genomics, psi, protein structure initiative, midwest center for structural genomics, mcsg, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 47195.56

構造登録者

Korolev, S.,Koroleva, O.,Petterson, K.,Collart, F.,Dementieva, I.,Joachimiak, A.,Midwest Center for Structural Genomics (MCSG) (登録日: 2001-10-15, 公開日: 2002-06-26, 最終更新日: 2024-02-07)

主引用文献

Korolev, S.,Koroleva, O.,Petterson, K.,Gu, M.,Collart, F.,Dementieva, I.,Joachimiak, A.
Autotracing of Escherichia coli acetate CoA-transferase alpha-subunit structure using 3.4 A MAD and 1.9 A native data.
Acta Crystallogr.,Sect.D, 58:2116-2121, 2002

PubMed Abstract: The automation of protein structure determination is an essential component for high-throughput structural analysis in protein X-ray crystallography and is a key element in structural genomics. This highly challenging undertaking relies at present on the availability of high-quality native and derivatized protein crystals diffracting to high or moderate resolution, respectively. Obtaining such crystals often requires significant effort. The present study demonstrates that phases obtained at low resolution (>3.0 A) from crystals of SeMet-labeled protein can be successfully used for automated structure determination. The crystal structure of acetate CoA-transferase alpha-subunit was solved using 3.4 A multi-wavelength anomalous dispersion data collected from a crystal containing SeMet-substituted protein and 1.9 A data collected from a native protein crystal.

PubMed: 12454473
DOI: 10.1107/S0907444902017055

実験手法

X-RAY DIFFRACTION (1.9 Å)