1K5W

THREE-DIMENSIONAL STRUCTURE OF THE SYNAPTOTAGMIN 1 C2B-DOMAIN: SYNAPTOTAGMIN 1 AS A PHOSPHOLIPID BINDING MACHINE

1K5W の概要

• エントリーDOI: 10.2210/pdb1k5w/pdb
• NMR情報: BMRB: 5194
• 分子名称: Synaptotagmin I, CALCIUM ION (2 entities in total)
• 機能のキーワード: c2b-domain, c2-domain, synaptotagmin i, calcium-binding, phospholipid-binding, synapsis, neurotransmitter release, synaptic vesicle exocytosis, endocytosis-exocytosis complex, endocytosis/exocytosis
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass membrane protein: P21707
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17432.36

構造登録者

Fernandez, I.,Arac, D.,Ubach, J.,Gerber, S.H.,Shin, O.,Gao, Y.,Anderson, R.G.W.,Sudhof, T.C.,Rizo, J. (登録日: 2001-10-12, 公開日: 2002-01-23, 最終更新日: 2024-05-22)

主引用文献

Fernandez, I.,Arac, D.,Ubach, J.,Gerber, S.H.,Shin, O.,Gao, Y.,Anderson, R.G.,Sudhof, T.C.,Rizo, J.
Three-dimensional structure of the synaptotagmin 1 C2B-domain: synaptotagmin 1 as a phospholipid binding machine.
Neuron, 32:1057-1069, 2001

PubMed Abstract: Synaptotagmin 1 probably functions as a Ca2+ sensor in neurotransmitter release via its two C2-domains, but no common Ca2+-dependent activity that could underlie a cooperative action between them has been described. The NMR structure of the C2B-domain now reveals a beta sandwich that exhibits striking similarities and differences with the C2A-domain. Whereas the bottom face of the C2B-domain has two additional alpha helices that may be involved in specialized Ca2+-independent functions, the top face binds two Ca2+ ions and is remarkably similar to the C2A-domain. Consistent with these results, but in contrast to previous studies, we find that the C2B-domain binds phospholipids in a Ca2+-dependent manner similarly to the C2A-domain. These results suggest a novel view of synaptotagmin function whereby the two C2-domains cooperate in a common activity, Ca2+-dependent phospholipid binding, to trigger neurotransmitter release.

PubMed: 11754837
DOI: 10.1016/S0896-6273(01)00548-7

実験手法

SOLUTION NMR