1K2V

E. COLI PERIPLASMIC PROTEIN FHUD COMPLEXED WITH DESFERAL

1K2V の概要

• エントリーDOI: 10.2210/pdb1k2v/pdb
• 関連するPDBエントリー: 1EFD 1ESZ
• 分子名称: Ferrichrome-binding periplasmic protein, DEFEROXAMINE MESYLATE FE(III) COMPLEX (3 entities in total)
• 機能のキーワード: two domains, alpha helix linker, metal transport
• 由来する生物種: Escherichia coli
• 細胞内の位置: Periplasm: P07822
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 30415.83

構造登録者

Clarke, T.E.,Braun, V.,Winkelmann, G.,Tari, L.W.,Vogel, H.J. (登録日: 2001-09-29, 公開日: 2002-04-17, 最終更新日: 2023-08-16)

主引用文献

Clarke, T.E.,Braun, V.,Winkelmann, G.,Tari, L.W.,Vogel, H.J.
X-ray crystallographic structures of the Escherichia coli periplasmic protein FhuD bound to hydroxamate-type siderophores and the antibiotic albomycin.
J.Biol.Chem., 277:13966-13972, 2002

PubMed Abstract: Siderophore-binding proteins play an essential role in the uptake of iron in many Gram-positive and Gram-negative bacteria. FhuD is an ATP-binding cassette-type (ABC-type) binding protein involved in the uptake of hydroxamate-type siderophores in Escherichia coli. Structures of FhuD complexed with the antibiotic albomycin, the fungal siderophore coprogen and the drug Desferal have been determined at high resolution by x-ray crystallography. FhuD has an unusual bilobal structure for a periplasmic ligand binding protein, with two mixed beta/alpha domains connected by a long alpha-helix. The binding site for hydroxamate-type ligands is composed of a shallow pocket that lies between these two domains. Recognition of siderophores primarily occurs through interactions between the iron-hydroxamate centers of each siderophore and the side chains of several key residues in the binding pocket. Rearrangements of side chains within the binding pocket accommodate the unique structural features of each siderophore. The backbones of the siderophores are not involved in any direct interactions with the protein, demonstrating how siderophores with considerable chemical and structural diversity can be bound by FhuD. For albomycin, which consists of an antibiotic group attached to a hydroxamate siderophore, electron density for the antibiotic portion was not observed. Therefore, this study provides a basis for the rational design of novel bacteriostatic agents, in the form of siderophore-antibiotic conjugates that can act as "Trojan horses," using the hydroxamate-type siderophore uptake system to actively deliver antibiotics directly into targeted pathogens.

PubMed: 11805094
DOI: 10.1074/jbc.M109385200

実験手法

X-RAY DIFFRACTION (1.97 Å)