1JZN

crystal structure of a galactose-specific C-type lectin

1JZN の概要

• エントリーDOI: 10.2210/pdb1jzn/pdb
• 関連するPDBエントリー: 1MUQ
• 関連するBIRD辞書のPRD_ID: PRD_900004
• 分子名称: Galactose-specific lectin, beta-D-galactopyranose-(1-4)-beta-D-glucopyranose, CALCIUM ION, ... (6 entities in total)
• 機能のキーワード: c-type lectin, protein-disaccharide complex, sugar binding protein
• 由来する生物種: Crotalus atrox (western diamondback rattlesnake)
• 細胞内の位置: Secreted: P21963
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 83624.16

構造登録者

Walker, J.R.,Nagar, B.,Young, N.M.,Hirama, T.,Rini, J.M. (登録日: 2001-09-16, 公開日: 2003-07-01, 最終更新日: 2024-11-06)

主引用文献

Walker, J.R.,Nagar, B.,Young, N.M.,Hirama, T.,Rini, J.M.
X-ray Crystal Structure of a Galactose-Specific C-Type Lectin Possessing a Novel Decameric Quaternary Structure.
Biochemistry, 43:3783-3792, 2004

PubMed Abstract: Rattlesnake venom lectin (RSL) from the western diamondback rattlesnake (Crotalus atrox) is an oligomeric galactose-specific C-type lectin. The X-ray crystal structure of RSL, in complex with lactose and thiodigalactoside, at 2.2 and 2.3 A resolution, respectively, reveals a decameric protein composed of two 5-fold symmetric pentamers arranged in a staggered, back-to-back orientation. Each monomer corresponds to a single canonical C-type lectin carbohydrate recognition domain devoid of accessory domains and is disulfide-bonded to a monomer in the other pentamer. The structure is the first example of that of a carbohydrate complex of a vertebrate galactose-specific C-type lectin. The 10 carbohydrate-binding sites, located on the rim of the decamer, suggest a role for multivalent interactions and a mechanism for RSL's ability to promote receptor cross-linking and cell aggregation.

PubMed: 15049685
DOI: 10.1021/bi035871a

実験手法

X-RAY DIFFRACTION (2.2 Å)