1JY3

Crystal Structure of the Central Region of Bovine Fibrinogen (E5 Fragment) at 1.4 Angstroms Resolution

1JY3 の概要

• エントリーDOI: 10.2210/pdb1jy3/pdb
• 関連するPDBエントリー: 1JY2
• 分子名称: FIBRINOGEN ALPHA CHAIN, FIBRINOGEN BETA CHAIN, FIBRINOGEN GAMMA-B CHAIN, ... (4 entities in total)
• 機能のキーワード: fibrinogen, fragment e, disulfide bonds, asymmetry, coiled-coil, beta-sheet, blood clotting
• 由来する生物種: Bos taurus (cattle); 詳細
• 細胞内の位置: Secreted : P02676 P12799
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 35804.29

構造登録者

Madrazo, J.,Brown, J.H.,Litvinovich, S.,Dominguez, R.,Yakovlev, S.,Medved, L.,Cohen, C. (登録日: 2001-09-10, 公開日: 2001-10-17, 最終更新日: 2024-11-13)

主引用文献

Madrazo, J.,Brown, J.H.,Litvinovich, S.,Dominguez, R.,Yakovlev, S.,Medved, L.,Cohen, C.
Crystal structure of the central region of bovine fibrinogen (E5 fragment) at 1.4-A resolution.
Proc.Natl.Acad.Sci.USA, 98:11967-11972, 2001

PubMed Abstract: The high-resolution crystal structure of the N-terminal central region of bovine fibrinogen (a 35-kDa E(5) fragment) reveals a remarkable dimeric design. The two halves of the molecule bond together at the center in an extensive molecular "handshake" by using both disulfide linkages and noncovalent contacts. On one face of the fragment, the Aalpha and Bbeta chains from the two monomers form a funnel-shaped domain with an unusual hydrophobic cavity; here, on each of the two outer sides there appears to be a binding site for thrombin. On the opposite face, the N-terminal gamma chains fold into a separate domain. Despite the chemical identity of the two halves of fibrinogen, an unusual pair of adjacent disulfide bonds locally constrain the two gamma chains to adopt different conformations. The striking asymmetry of this domain may promote the known supercoiling of the protofibrils in fibrin. This information on the detailed topology of the E(5) fragment permits the construction of a more detailed model than previously possible for the critical trimolecular junction of the protofibril in fibrin.

PubMed: 11593005
DOI: 10.1073/pnas.211439798

実験手法

X-RAY DIFFRACTION (1.6 Å)