1JXG

The 1.6 A Resolution Crystal Structure of a Mutant Poplar Plastocyanin Bearing a 21-25 Engeneered Disulfide Bridge

1JXG の概要

• エントリーDOI: 10.2210/pdb1jxg/pdb
• 分子名称: PLASTOCYANIN A, COPPER (I) ION, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: beta barrel, photosynthesis
• 由来する生物種: Populus nigra
• 細胞内の位置: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Lumenal side: P00299
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21400.79

構造登録者

Milani, M.,Andolfi, L.,Cannistraro, S.,Verbeet, M.P.,Bolognesi, M. (登録日: 2001-09-07, 公開日: 2001-12-19, 最終更新日: 2024-11-06)

主引用文献

Milani, M.,Andolfi, L.,Cannistraro, S.,Verbeet, M.P.,Bolognesi, M.
The 1.6 A resolution crystal structure of a mutant plastocyanin bearing a 21-25 engineered disulfide bridge.
Acta Crystallogr.,Sect.D, 57:1735-1738, 2001

PubMed Abstract: Plastocyanin is an electron-transfer protein which has been largely used for biophysical studies as well as for protein-engineering experiments. A surface disulfide bridge has been engineered in poplar plastocyanin to allow protein chemisorption on gold substrates. The mutated plastocyanin crystal structure has been studied at 1.6 A resolution (R factor = 0.145, R(free) = 0.205) to characterize the effects of the engineered disulfide on the overall protein structure and on the Cu-coordination sphere in view of biophysical applications. The new orthorhombic crystal form isolated for the mutated plastocyanin displays two protein molecules per asymmetric unit.

PubMed: 11679761
DOI: 10.1107/S0907444901013221

実験手法

X-RAY DIFFRACTION (1.6 Å)