1JXA

GLUCOSAMINE 6-PHOSPHATE SYNTHASE WITH GLUCOSE 6-PHOSPHATE

1JXA の概要

• エントリーDOI: 10.2210/pdb1jxa/pdb
• 関連するPDBエントリー: 1gdo 1moq
• 分子名称: glucosamine 6-phosphate synthase, GLUCOSE-6-PHOSPHATE (3 entities in total)
• 機能のキーワード: beta-sandwich, nucleotide-binding fold, gene duplication, ammonia channel, transferase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 200974.27

構造登録者

Teplyakov, A.,Obmolova, G.,Badet, B.,Badet-Denisot, M.A. (登録日: 2001-09-06, 公開日: 2001-11-21, 最終更新日: 2024-12-25)

主引用文献

Teplyakov, A.,Obmolova, G.,Badet, B.,Badet-Denisot, M.A.
Channeling of ammonia in glucosamine-6-phosphate synthase.
J.Mol.Biol., 313:1093-1102, 2001

PubMed Abstract: Glucosamine-6-phosphate synthase catalyses the first and rate-limiting step in hexosamine metabolism, converting fructose 6-phosphate into glucosamine 6-phosphate in the presence of glutamine. The crystal structure of the Escherichia coli enzyme reveals the domain organisation of the homodimeric molecule. The 18 A hydrophobic channel sequestered from the solvent connects the glutaminase and isomerase active sites, and provides a means of ammonia transfer from glutamine to sugar phosphate. The C-terminal decapeptide sandwiched between the two domains plays a central role in the transfer. Based on the structure, a mechanism of enzyme action and self-regulation is proposed. It involves large domain movements triggered by substrate binding that lead to the formation of the channel.

PubMed: 11700065
DOI: 10.1006/jmbi.2001.5094

実験手法

X-RAY DIFFRACTION (3.1 Å)