1JVB

ALCOHOL DEHYDROGENASE FROM THE ARCHAEON SULFOLOBUS SOLFATARICUS

1JVB の概要

• エントリーDOI: 10.2210/pdb1jvb/pdb
• 分子名称: NAD(H)-DEPENDENT ALCOHOL DEHYDROGENASE, ZINC ION (3 entities in total)
• 機能のキーワード: archaeon, zinc, nad, oxidoreductase
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 38121.50

構造登録者

Esposito, L.,Sica, F.,Zagari, A.,Mazzarella, L. (登録日: 2001-08-29, 公開日: 2002-08-29, 最終更新日: 2024-11-13)

主引用文献

Esposito, L.,Sica, F.,Raia, C.A.,Giordano, A.,Rossi, M.,Mazzarella, L.,Zagari, A.
Crystal structure of the alcohol dehydrogenase from the hyperthermophilic archaeon Sulfolobus solfataricus at 1.85 A resolution.
J.Mol.Biol., 318:463-477, 2002

PubMed Abstract: The crystal structure of a medium-chain NAD(H)-dependent alcohol dehydrogenase (ADH) from an archaeon has been solved by multiwavelength anomalous diffraction, using a selenomethionine-substituted enzyme. The protein (SsADH), extracted from the hyperthermophilic organism Sulfolobus solfataricus, is a homo-tetramer with a crystallographic 222 symmetry. Despite the low level of sequence identity, the overall fold of the monomer is similar to that of the other homologous ADHs of known structure. However, a significant difference is the orientation of the catalytic domain relative to the coenzyme-binding domain that results in a larger interdomain cleft. At the bottom of this cleft, the catalytic zinc ion is coordinated tetrahedrally and lacks the zinc-bound water molecule that is usually found in ADH apoform structures. The fourth coordination position is indeed occupied by a Glu residue, as found in bacterial tetrameric ADHs. Other differences are found in the architecture of the substrate pocket whose entrance is more restricted than in other ADHs. SsADH is the first tetrameric ADH X-ray structure containing a second zinc ion playing a structural role. This latter metal ion shows a peculiar coordination, with a glutamic acid residue replacing one of the four cysteine ligands that are highly conserved throughout the structural zinc-containing dimeric ADHs.

PubMed: 12051852
DOI: 10.1016/S0022-2836(02)00088-8

実験手法

X-RAY DIFFRACTION (1.85 Å)