1JU2

Crystal structure of the hydroxynitrile lyase from almond

1JU2 の概要

• エントリーDOI: 10.2210/pdb1ju2/pdb
• 分子名称: hydroxynitrile lyase, 2-acetamido-2-deoxy-alpha-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (10 entities in total)
• 機能のキーワード: hydroxynitrile lyase, flavin, gmc oxidoreductase, almond, cyanogenesis, lyase
• 由来する生物種: Prunus dulcis (almond)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 122581.05

構造登録者

Dreveny, I.,Gruber, K.,Glieder, A.,Thompson, A.,Kratky, C. (登録日: 2001-08-23, 公開日: 2002-09-04, 最終更新日: 2024-10-23)

主引用文献

Dreveny, I.,Gruber, K.,Glieder, A.,Thompson, A.,Kratky, C.
The hydroxynitrile lyase from almond: a lyase that looks like an oxidoreductase.
Structure, 9:803-815, 2001

PubMed Abstract: Cyanogenesis is a defense process of several thousand plant species. Hydroxynitrile lyase, a key enzyme of this process, cleaves a cyanohydrin into hydrocyanic acid and the corresponding aldehyde or ketone. The reverse reaction constitutes an important tool in biocatalysis. Different classes of hydroxynitrile lyases have convergently evolved from FAD-dependent oxidoreductases, alpha/beta hydrolases, and alcohol dehydrogenases. The FAD-dependent hydroxynitrile lyases (FAD-HNLs) carry a flavin cofactor whose redox properties appear to be unimportant for catalysis.

PubMed: 11566130
DOI: 10.1016/S0969-2126(01)00639-6

実験手法

X-RAY DIFFRACTION (1.47 Å)