1JRO

Crystal Structure of Xanthine Dehydrogenase from Rhodobacter capsulatus

1JRO の概要

• エントリーDOI: 10.2210/pdb1jro/pdb
• 関連するPDBエントリー: 1JRP
• 分子名称: xanthine dehydrogenase, chain A, xanthine dehydrogenase, chain B, FE2/S2 (INORGANIC) CLUSTER, ... (8 entities in total)
• 機能のキーワード: partial beta-barrel; xdh; xo, oxidoreductase
• 由来する生物種: Rhodobacter capsulatus; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 536236.46

構造登録者

Truglio, J.J.,Theis, K.,Leimkuhler, S.,Rappa, R.,Rajagopalan, K.V.,Kisker, C. (登録日: 2001-08-14, 公開日: 2002-01-11, 最終更新日: 2023-08-16)

主引用文献

Truglio, J.J.,Theis, K.,Leimkuhler, S.,Rappa, R.,Rajagopalan, K.V.,Kisker, C.
Crystal structures of the active and alloxanthine-inhibited forms of xanthine dehydrogenase from Rhodobacter capsulatus
Structure, 10:115-125, 2002

PubMed Abstract: Xanthine dehydrogenase (XDH), a complex molybdo/iron-sulfur/flavoprotein, catalyzes the oxidation of hypoxanthine to xanthine followed by oxidation of xanthine to uric acid with concomitant reduction of NAD+. The 2.7 A resolution structure of Rhodobacter capsulatus XDH reveals that the bacterial and bovine XDH have highly similar folds despite differences in subunit composition. The NAD+ binding pocket of the bacterial XDH resembles that of the dehydrogenase form of the bovine enzyme rather than that of the oxidase form, which reduces O(2) instead of NAD+. The drug allopurinol is used to treat XDH-catalyzed uric acid build-up occurring in gout or during cancer chemotherapy. As a hypoxanthine analog, it is oxidized to alloxanthine, which cannot be further oxidized but acts as a tight binding inhibitor of XDH. The 3.0 A resolution structure of the XDH-alloxanthine complex shows direct coordination of alloxanthine to the molybdenum via a nitrogen atom. These results provide a starting point for the rational design of new XDH inhibitors.

PubMed: 11796116
DOI: 10.1016/S0969-2126(01)00697-9

実験手法

X-RAY DIFFRACTION (2.7 Å)