1JQL

Mechanism of Processivity Clamp Opening by the Delta Subunit Wrench of the Clamp Loader Complex of E. coli DNA Polymerase III: Structure of beta-delta (1-140)

1JQL の概要

• エントリーDOI: 10.2210/pdb1jql/pdb
• 関連するPDBエントリー: 1JQJ 1JR3 1a5t 2pol
• 分子名称: DNA Polymerase III, BETA CHAIN, DNA Polymerase III, DELTA SUBUNIT (2 entities in total)
• 機能のキーワード: dna polymerase, processivity clamp, clamp loader, dna replication, aaa+ atpase, transferase
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm : P0A988
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56113.03

構造登録者

Jeruzalmi, D.,Yurieva, O.,Zhao, Y.,Young, M.,Stewart, J.,Hingorani, M.,O'Donnell, M.,Kuriyan, J. (登録日: 2001-08-07, 公開日: 2001-09-26, 最終更新日: 2023-11-29)

主引用文献

Jeruzalmi, D.,Yurieva, O.,Zhao, Y.,Young, M.,Stewart, J.,Hingorani, M.,O'Donnell, M.,Kuriyan, J.
Mechanism of processivity clamp opening by the delta subunit wrench of the clamp loader complex of E. coli DNA polymerase III.
Cell(Cambridge,Mass.), 106:417-428, 2001

PubMed Abstract: The dimeric ring-shaped sliding clamp of E. coli DNA polymerase III (beta subunit, homolog of eukaryotic PCNA) is loaded onto DNA by the clamp loader gamma complex (homolog of eukaryotic Replication Factor C, RFC). The delta subunit of the gamma complex binds to the beta ring and opens it. The crystal structure of a beta:delta complex shows that delta, which is structurally related to the delta' and gamma subunits of the gamma complex, is a molecular wrench that induces or traps a conformational change in beta such that one of its dimer interfaces is destabilized. Structural comparisons and molecular dynamics simulations suggest a spring-loaded mechanism in which the beta ring opens spontaneously once a dimer interface is perturbed by the delta wrench.

PubMed: 11525728
DOI: 10.1016/S0092-8674(01)00462-7

実験手法

X-RAY DIFFRACTION (2.5 Å)