1JP9

Sperm Whale met-Myoglobin (low temperature; high pressure)

1JP9 の概要

• エントリーDOI: 10.2210/pdb1jp9/pdb
• 関連するPDBエントリー: 1A6K 1JP6 1JP8 1JPB
• 分子名称: myoglobin, SULFATE ION, PROTOPORPHYRIN IX CONTAINING FE, ... (4 entities in total)
• 機能のキーワード: oxygen storage/transport, oxygen storage-transport complex
• 由来する生物種: Physeter catodon (sperm whale)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 18331.75

構造登録者

Urayama, P.,Gruner, S.M.,Phillips Jr., G.N. (登録日: 2001-08-01, 公開日: 2002-01-16, 最終更新日: 2023-08-16)

主引用文献

Urayama, P.,Phillips Jr., G.N.,Gruner, S.M.
Probing substates in sperm whale myoglobin using high-pressure crystallography.
Structure, 10:51-60, 2002

PubMed Abstract: Pressures in the 100 MPa range are known to have an enormous number of effects on the action of proteins, but straightforward means for determining the structural basis of these effects have been lacking. Here, crystallography has been used to probe effects of pressure on sperm whale myoglobin structure. A comparison of pressure effects with those seen at low pH suggests that structural changes under pressure are interpretable as a shift in the populations of conformational substates. Furthermore, a novel high-pressure protein crystal-cooling method has been used to show low-temperature metastability, providing an alternative to room temperature, beryllium pressure cell-based techniques. The change in protein structure due to pressure is not purely compressive and involves conformational changes important to protein activity. Correlation with low-pH structures suggests observed structural changes are associated with global conformational substates. Methods developed here open up a direct avenue for exploration of the effects of pressure on proteins.

PubMed: 11796110
DOI: 10.1016/S0969-2126(01)00699-2

実験手法

X-RAY DIFFRACTION (1.7 Å)