1JP0

NMR Structure of the LP5.1 Hairpin from Bacillus RNase P RNA Refined WITHOUT Residual Dipolar Couplings

1JP0 の概要

• エントリーDOI: 10.2210/pdb1jp0/pdb
• 関連するPDBエントリー: 1JOX
• 分子名称: 5'-R(*GP*GP*CP*GP*GP*UP*GP*CP*UP*GP*AP*GP*AP*UP*GP*CP*CP*CP*GP*UP*C)-3' (1 entity in total)
• 機能のキーワード: ribonucleic acid, rnase p, hairpin, residual dipolar coupling refinement, ugnrau, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6776.06

構造登録者

Leeper, T.C.,Schmidt, F.J.,Van Doren, S.R. (登録日: 2001-07-31, 公開日: 2002-05-01, 最終更新日: 2024-05-22)

主引用文献

Leeper, T.C.,Martin, M.B.,Kim, H.,Cox, S.,Semenchenko, V.,Schmidt, F.J.,Van Doren, S.R.
Structure of the UGAGAU hexaloop that braces Bacillus RNase P for action.
Nat.Struct.Biol., 9:397-403, 2002

PubMed Abstract: Long-range interactions involving the P5.1 hairpin of Bacillus RNase P RNA are thought to form a structural truss to support RNA folding and activity. We determined the structure of this element by NMR and refined the structure using residual dipolar couplings from a sample weakly oriented in a dilute liquid crystalline mixture of polyethylene glycol and hexanol. Dipolar coupling refinement improved the global precision of the structure from 1.5 to 1.2 A (to the mean), revised the bend angle between segments of the P5.1 stem and corroborated the structure of the loop region. The UGAGAU hexaloop of P5.1 contains two stacks of bases on opposite sides of the loop, distinguishing it from GNRA tetraloops. The unusual conformation of the juxtaposed uracil residues within the hexaloop may explain their requirement in transactivation assays.

PubMed: 11927952

実験手法

SOLUTION NMR