1JNL

Crystal Structure of Fab-Estradiol Complexes

1JNL の概要

• エントリーDOI: 10.2210/pdb1jnl/pdb
• 分子名称: monoclonal anti-estradiol 17E12E5 immunoglobulin kappa chain, monoclonal anti-estradiol 17E12E5 immunoglobulin gamma-1 chain (2 entities in total)
• 機能のキーワード: igg fold, antibody-hapten complex, estradiol, immune system
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 46589.94

構造登録者

Monnet, C.,Bettsworth, F.,Stura, E.A.,Le Du, M.-H.,Menez, R.,Derrien, L.,Zinn-Justin, S.,Gilquin, B.,Sibai, G.,Battail-Poirot, N.,Jolivet, M.,Menez, A.,Arnaud, M.,Ducancel, F.,Charbonnier, J.B. (登録日: 2001-07-24, 公開日: 2002-02-06, 最終更新日: 2024-10-30)

主引用文献

Monnet, C.,Bettsworth, F.,Stura, E.A.,Du, M.H.,Menez, R.,Derrien, L.,Zinn-Justin, S.,Gilquin, B.,Sibai, G.,Battail-Poirot, N.,Jolivet, M.,Menez, A.,Arnaud, M.,Ducancel, F.,Charbonnier, J.B.
Highly specific anti-estradiol antibodies: structural characterisation and binding diversity.
J.Mol.Biol., 315:699-712, 2002

PubMed Abstract: Subtle modulation of antibody-binding properties by protein engineering often lies with an accurate structural and energetic description of how an antigen is recognised. Thus, with the intent to increase the affinity and add a bias in favour of natural estradiol compared with its chemically modified immunogen, we have determined the crystal structure of two anti-estradiol monoclonal antibodies, 10G6D6 and 17E12E5. Although generated against the same estradiol derivative, these antibodies share little sequence identity, which is reflected in dissimilar binding pockets and in different positioning of the steroid. In both antibodies the characteristic 17-hydroxyl group is buried deeply at the bottom of hydrophobic pockets and stabilised by hydrogen bonds. Apart from this similarity, the steroid is oriented differently in the respective binding pockets. The high specificity of both antibodies has been mapped out, and even closely related steroids show low cross-reactivity. The structural studies of the complex formed between 10G6D6 and 6-CMO-estradiol have identified contacts between the 6-CMO coupling linker and an arginine residue from the heavy chain CDR2 segment. This segment is now being targeted by random mutagenesis to select mutants with a preference for natural estradiol compared to the branched hapten.

PubMed: 11812141
DOI: 10.1006/jmbi.2001.5284

実験手法

X-RAY DIFFRACTION (3 Å)