1JMM

Crystal structure of the V-region of Streptococcus mutans antigen I/II

1JMM の概要

• エントリーDOI: 10.2210/pdb1jmm/pdb
• 分子名称: protein I/II V-region, SODIUM ION (3 entities in total)
• 機能のキーワード: antigen i/ii, v-region, immune system, membrane protein
• 由来する生物種: Streptococcus mutans
• 細胞内の位置: Secreted, cell wall; Peptidoglycan-anchor (Potential): P11657
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 41638.11

構造登録者

Troffer-Charlier, N.,Ogier, J.,Moras, D.,Cavarelli, J. (登録日: 2001-07-19, 公開日: 2002-07-17, 最終更新日: 2011-07-13)

主引用文献

Troffer-Charlier, N.,Ogier, J.,Moras, D.,Cavarelli, J.
Crystal Structure of the V-region of Streptococcus mutans Antigen I/II at 2.4 a Resolution Suggests a Sugar Preformed Binding Site
J.Mol.Biol., 318:179-188, 2002

PubMed Abstract: Antigens I/II are large multifunctional adhesins from oral viridans streptococci that exert immunomodulatory effects on human cells and play important roles in inflammatory disorders. Among them, Streptococcus mutans plays a major role in the initiation of dental caries. The structure of the V-region (SrV+, residues 464-840) of the antigen I/II of S. mutans has been determined using the multiwavelength anomalous diffraction phasing technique with seleno-methionine-substituted recombinant protein and subsequently refined at 2.4 A resolution. The crystal structure of SrV+ revealed a lectin-like fold that displays a putative preformed carbohydrate-binding site stabilized by a metal ion. Inhibition of this binding site may confer to humans a protection against dental caries and dissemination of the bacteria to extra-oral sites involved in life-threatening inflammatory diseases. This crystal structure constitutes a first step in understanding the structure-function relationship of antigens I/II and may help in delineating new preventive or therapeutic strategies against colonization of the host by oral streptococci.

PubMed: 12054777
DOI: 10.1016/S0022-2836(02)00025-6

実験手法

X-RAY DIFFRACTION (2.4 Å)