1JJH

E2 DNA-binding Domain from Bovine Papillomavirus Type 1

1JJH の概要

• エントリーDOI: 10.2210/pdb1jjh/pdb
• 関連するPDBエントリー: 1by9 1f9f 1jj4 2bop
• 分子名称: REGULATORY PROTEIN E2 (2 entities in total)
• 機能のキーワード: bpv-1, e2, dna-binding domain, dna binding protein
• 由来する生物種: Bovine papillomavirus type 1
• 細胞内の位置: Host nucleus : P03122
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 29075.90

構造登録者

Hegde, R.S.,Wang, A.F.,Kim, S.S.,Schapira, M. (登録日: 2001-07-05, 公開日: 2001-07-18, 最終更新日: 2024-11-13)

主引用文献

Hegde, R.S.,Wang, A.F.,Kim, S.S.,Schapira, M.
Subunit rearrangement accompanies sequence-specific DNA binding by the bovine papillomavirus-1 E2 protein.
J.Mol.Biol., 276:797-808, 1998

PubMed Abstract: The 2.5 A crystal structures of the DNA-binding domain of the E2 protein from bovine papillomavirus strain 1 and its complex with DNA are presented. E2 is a transcriptional regulatory protein that is also involved in viral DNA replication. It is the structural prototype for a novel class of DNA-binding proteins: dimeric beta-barrels with surface alpha-helices that serve as recognition helices. These helices contain the amino-acid residues involved in sequence-specifying interactions. The E2 proteins from different papillomavirus strains recognize and bind to the same consensus 12 base-pair DNA sequence. However, recent evidence from solution studies points to differences in the mechanisms by which E2 from the related viral strains bovine papillomavirus-1 and human papillomavirus-16 discriminate between DNA targets based on non-contacted nucleotide sequences. This report provides evidence that sequence-specific DNA-binding is accompanied by a rearrangement of protein subunits and deformation of the DNA. These results suggest that, along with DNA sequence-dependent conformational properties, protein subunit orientation plays a significant role in the mechanisms of target selection utilized by E2.

PubMed: 9500927
DOI: 10.1006/jmbi.1997.1587

実験手法

X-RAY DIFFRACTION (2.5 Å)