1JIW

Crystal structure of the APR-APRin complex

1JIW の概要

• エントリーDOI: 10.2210/pdb1jiw/pdb
• 関連するPDBエントリー: 1SMP
• 分子名称: ALKALINE METALLOPROTEINASE, PROTEINASE INHIBITOR, ZINC ION, ... (5 entities in total)
• 機能のキーワード: pseudomonas aeruginosa alkaline protease inhibitor, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Pseudomonas aeruginosa; 詳細
• 細胞内の位置: Secreted: Q03023; Periplasm: Q03026
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 61314.28

構造登録者

Hege, T.,Feltzer, R.E.,Gray, R.D.,Baumann, U. (登録日: 2001-07-03, 公開日: 2001-08-15, 最終更新日: 2024-11-20)

主引用文献

Hege, T.,Feltzer, R.E.,Gray, R.D.,Baumann, U.
Crystal structure of a complex between Pseudomonas aeruginosa alkaline protease and its cognate inhibitor: inhibition by a zinc-NH2 coordinative bond
J.Biol.Chem., 276:35087-35092, 2001

PubMed Abstract: Serralysins are a family of metalloproteases secreted by Gram-negative bacteria into the medium in the form of inactive zymogens. Usually, all serralysin secretors have on the same operon a gene coding for a periplasmic 10-kDa protein, which is an inhibitor of the secreted protease. The recent characterization of the inhibitor of the alkaline protease from Pseudomonas aeruginosa revealed a surprisingly low dissociation constant of 4 pm, contrary to earlier studies on homologous systems, where inhibition constants in the microm range were reported. To approach a more accurate understanding, the crystal structure of the complex between inhibitor and protease from P. aeruginosa was determined at 1.74 A resolution and refined to R(free) = 0.204. The structure reported here shows clearly that the N terminus of the inhibitor forms a coordinative bond to the catalytic Zn(2+) ion with a nitrogen-zinc distance of 2.17 A. We conclude that this interaction adds substantially to the complex stability and show also that similar interactions are found in other metzincin-inhibitor complexes.

PubMed: 11445573
DOI: 10.1074/jbc.M104020200

実験手法

X-RAY DIFFRACTION (1.74 Å)