1JIH

Yeast DNA Polymerase ETA

Summary for 1JIH

• Entry DOI: 10.2210/pdb1jih/pdb
• Descriptor: DNA Polymerase ETA (2 entities in total)
• Functional Keywords: dna polymerase, translesion, yeast, translation
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Nucleus: Q04049
• Total number of polymer chains: 2
• Total formula weight: 116307.50

Authors

Trincao, J.,Johnson, R.E.,Escalante, C.R.,Prakash, S.,Prakash, L.,Aggarwal, A.K. (deposition date: 2001-07-02, release date: 2002-01-09, Last modification date: 2024-02-07)

Primary citation

Trincao, J.,Johnson, R.E.,Escalante, C.R.,Prakash, S.,Prakash, L.,Aggarwal, A.K.
Structure of the catalytic core of S. cerevisiae DNA polymerase eta: implications for translesion DNA synthesis
Mol.Cell, 8:417-426, 2001

PubMed Abstract: DNA polymerase eta is unique among eukaryotic polymerases in its proficient ability to replicate through a variety of distorting DNA lesions. We report here the crystal structure of the catalytic core of S. cerevisiae DNA polymerase eta, determined at 2.25A resolution. The structure reveals a novel polydactyl right hand-shaped molecule with a unique polymerase-associated domain. We identify the catalytic residues and show that the fingers and thumb domains are unusually small and stubby. In particular, the unexpected absence of helices "O" and "O1" in the fingers domain suggests that openness of the active site is the critical feature which enables DNA polymerase eta to replicate through DNA lesions such as a UV-induced cis-syn thymine-thymine dimer.

PubMed: 11545743
DOI: 10.1016/S1097-2765(01)00306-9

Experimental method

X-RAY DIFFRACTION (2.25 Å)