1JIH

Yeast DNA Polymerase ETA

1JIH の概要

• エントリーDOI: 10.2210/pdb1jih/pdb
• 分子名称: DNA Polymerase ETA (2 entities in total)
• 機能のキーワード: dna polymerase, translesion, yeast, translation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: Q04049
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 116307.50

構造登録者

Trincao, J.,Johnson, R.E.,Escalante, C.R.,Prakash, S.,Prakash, L.,Aggarwal, A.K. (登録日: 2001-07-02, 公開日: 2002-01-09, 最終更新日: 2024-02-07)

主引用文献

Trincao, J.,Johnson, R.E.,Escalante, C.R.,Prakash, S.,Prakash, L.,Aggarwal, A.K.
Structure of the catalytic core of S. cerevisiae DNA polymerase eta: implications for translesion DNA synthesis
Mol.Cell, 8:417-426, 2001

PubMed Abstract: DNA polymerase eta is unique among eukaryotic polymerases in its proficient ability to replicate through a variety of distorting DNA lesions. We report here the crystal structure of the catalytic core of S. cerevisiae DNA polymerase eta, determined at 2.25A resolution. The structure reveals a novel polydactyl right hand-shaped molecule with a unique polymerase-associated domain. We identify the catalytic residues and show that the fingers and thumb domains are unusually small and stubby. In particular, the unexpected absence of helices "O" and "O1" in the fingers domain suggests that openness of the active site is the critical feature which enables DNA polymerase eta to replicate through DNA lesions such as a UV-induced cis-syn thymine-thymine dimer.

PubMed: 11545743
DOI: 10.1016/S1097-2765(01)00306-9

実験手法

X-RAY DIFFRACTION (2.25 Å)