1JHS

Protein Mog1 E65A mutant

1JHS の概要

• エントリーDOI: 10.2210/pdb1jhs/pdb
• 分子名称: MOG1 PROTEIN (2 entities in total)
• 機能のキーワード: nuclear-protein import, gsp1, gene regulation
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P47123
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21015.56

構造登録者

Baker, R.P.,Harreman, M.T.,Ecclestone, J.F.,Corbett, A.H.,Stewart, M. (登録日: 2001-06-28, 公開日: 2003-06-03, 最終更新日: 2024-04-03)

主引用文献

Baker, R.P.,Harreman, M.T.,Ecclestone, J.F.,Corbett, A.H.,Stewart, M.
Interaction between Ran and Mog1 is required for efficient nuclear protein import
J.Biol.Chem., 276:41255-41262, 2001

PubMed Abstract: Mog1 is a nuclear protein that interacts with Ran, the Ras family GTPase that confers directionality to nuclear import and export pathways. Deletion of MOG1 in Saccharomyces cerevisiae (Deltamog1) causes temperature-sensitive growth and defects in nuclear protein import. Mog1 has previously been shown to stimulate GTP release from Ran and we demonstrate here that addition of Mog1 to either Ran-GTP or Ran-GDP results in nucleotide release and formation of a stable complex between Mog1 and nucleotide-free Ran. Moreover, MOG1 shows synthetic lethality with PRP20, the Ran guanine nucleotide exchange factor (RanGEF) that also binds nucleotide-free Ran. To probe the functional role of the Mog1-Ran interaction, we engineered mutants of yeast Mog1 and Ran that specifically disrupt their interaction both in vitro and in vivo. These mutants indicate that the interaction interface involves conserved Mog1p residues Asp(62) and Glu(65), and residue Lys(136) in yeast Ran. Mutations at these residues decrease the ability of Mog1 to bind and release nucleotide from Ran. Furthermore, the E65K-Mog1 and K136E-Ran mutations in yeast cause temperature sensitivity and mislocalization of a nuclear import reporter protein, similar to the phenotype observed for the Deltamog1 strain. Our results indicate that a primary function of Mog1 requires binding to Ran and that the Mog1-Ran interaction is necessary for efficient nuclear protein import in vivo.

PubMed: 11509570
DOI: 10.1074/jbc.M106060200

実験手法

X-RAY DIFFRACTION (1.9 Å)