1JGO

The Path of Messenger RNA Through the Ribosome. THIS FILE, 1JGO, CONTAINS THE 30S RIBOSOME SUBUNIT, THREE TRNA, AND MRNA MOLECULES. 50S RIBOSOME SUBUNIT IS IN THE FILE 1GIY

1JGO の概要

• エントリーDOI: 10.2210/pdb1jgo/pdb
• 関連するPDBエントリー: 1GIX 1GIY 1JGO 1JGP 1JGQ
• 分子名称: 30S 16S ribosomal RNA, 30S RIBOSOMAL PROTEIN S7, 30S RIBOSOMAL PROTEIN S8, ... (24 entities in total)
• 機能のキーワード: ribosome assembly, protein synthesis, life, ribosome
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 25
• 化学式量合計: 866197.90

構造登録者

Yusupova, G.Z.,Yusupov, M.M.,Cate, J.H.D.,Noller, H.F. (登録日: 2001-06-26, 公開日: 2001-07-20, 最終更新日: 2024-05-22)

主引用文献

Yusupova, G.Z.,Yusupov, M.M.,Cate, J.H.,Noller, H.F.
The path of messenger RNA through the ribosome.
Cell(Cambridge,Mass.), 106:233-241, 2001

PubMed Abstract: Using X-ray crystallography, we have directly observed the path of mRNA in the 70S ribosome in Fourier difference maps at 7 A resolution. About 30 nucleotides of the mRNA are wrapped in a groove that encircles the neck of the 30S subunit. The Shine-Dalgarno helix is bound in a large cleft between the head and the back of the platform. At the interface, only about eight nucleotides (-1 to +7), centered on the junction between the A and P codons, are exposed, and bond almost exclusively to 16S rRNA. The mRNA enters the ribosome around position +13 to +15, the location of downstream pseudoknots that stimulate -1 translational frame shifting.

PubMed: 11511350
DOI: 10.1016/S0092-8674(01)00435-4

実験手法

X-RAY DIFFRACTION (5.6 Å)