1JGJ

CRYSTAL STRUCTURE OF SENSORY RHODOPSIN II AT 2.4 ANGSTROMS: INSIGHTS INTO COLOR TUNING AND TRANSDUCER INTERACTION

1JGJ の概要

• エントリーDOI: 10.2210/pdb1jgj/pdb
• 関連するPDBエントリー: 1c3w
• 分子名称: SENSORY RHODOPSIN II, octyl beta-D-glucopyranoside, RETINAL, ... (4 entities in total)
• 機能のキーワード: sensory rhodopsin, membrane protein, phototaxis receptor, signaling protein
• 由来する生物種: Natronomonas pharaonis
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: P42196
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 23752.28

構造登録者

Luecke, H. (登録日: 2001-06-25, 公開日: 2001-07-18, 最終更新日: 2024-10-16)

主引用文献

Luecke, H.,Schobert, B.,Lanyi, J.K.,Spudich, E.N.,Spudich, J.L.
Crystal structure of sensory rhodopsin II at 2.4 angstroms: insights into color tuning and transducer interaction.
Science, 293:1499-1503, 2001

PubMed Abstract: We report an atomic-resolution structure for a sensory member of the microbial rhodopsin family, the phototaxis receptor sensory rhodopsin II (NpSRII), which mediates blue-light avoidance by the haloarchaeon Natronobacterium pharaonis. The 2.4 angstrom structure reveals features responsible for the 70- to 80-nanometer blue shift of its absorption maximum relative to those of haloarchaeal transport rhodopsins, as well as structural differences due to its sensory, as opposed to transport, function. Multiple factors appear to account for the spectral tuning difference with respect to bacteriorhodopsin: (i) repositioning of the guanidinium group of arginine 72, a residue that interacts with the counterion to the retinylidene protonated Schiff base; (ii) rearrangement of the protein near the retinal ring; and (iii) changes in tilt and slant of the retinal polyene chain. Inspection of the surface topography reveals an exposed polar residue, tyrosine 199, not present in bacteriorhodopsin, in the middle of the membrane bilayer. We propose that this residue interacts with the adjacent helices of the cognate NpSRII transducer NpHtrII.

PubMed: 11452084
DOI: 10.1126/science.1062977

実験手法

X-RAY DIFFRACTION (2.4 Å)