1JGD

HLA-B*2709 bound to deca-peptide s10R

1JGD の概要

• エントリーDOI: 10.2210/pdb1jgd/pdb
• 関連するPDBエントリー: 1hsa 1jge 1k5n
• 分子名称: HUMAN LYMPHOCYTE ANTIGEN HLA-B27, BETA-2-MICROGLOBULIN, peptide s10R, ... (5 entities in total)
• 機能のキーワード: mhc (major histocompatibility complex), hla (human leukocyte antigen), immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P03989; Secreted . Note=(Microbial infection) In the presence of M: P61769
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 45239.18

構造登録者

Hillig, R.C.,Huelsmeyer, M.,Saenger, W.,Volz, A.,Uchanska-Ziegler, B.,Ziegler, A. (登録日: 2001-06-25, 公開日: 2003-07-01, 最終更新日: 2024-10-09)

主引用文献

Hillig, R.C.,Huelsmeyer, M.,Saenger, W.,Welfle, K.,Misselwitz, R.,Welfle, H.,Kozerski, C.,Volz, A.,Uchanska-Ziegler, B.,Ziegler, A.
Thermodynamic and structural analysis of peptide- and allele-dependent properties of two HLA-B27 subtypes exhibiting differential disease association
J.Biol.Chem., 279:652-663, 2004

PubMed Abstract: Selected HLA-B27 subtypes are associated with spondyloarthropathies, but the underlying mechanism is not understood. To explain this association in molecular terms, a comparison of peptide-dependent dynamic and structural properties of the differentially disease-associated subtypes HLA-B*2705 and HLA-B*2709 was carried out. These molecules differ only by a single amino acid at the floor of the peptide binding groove. The thermostabilities of a series of HLA-B27 molecules complexed with nonameric and decameric peptides were determined and revealed substantial differences depending on the subtype as well as the residues at the termini of the peptides. In addition we present the crystal structure of the B*2709 subtype complexed with a decameric peptide. This structure provides an explanation for the preference of HLA-B27 for a peptide with an N-terminal arginine as secondary anchor and the lack of preference for tyrosine as peptide C terminus in B*2709. The data show that differences in thermodynamic properties between peptide-complexed HLA-B27 subtypes are correlated with a variety of structural properties.

PubMed: 14555655
DOI: 10.1074/jbc.M307457200

実験手法

X-RAY DIFFRACTION (1.9 Å)