1JFI

Crystal Structure of the NC2-TBP-DNA Ternary Complex

Summary for 1JFI

• Entry DOI: 10.2210/pdb1jfi/pdb
• Descriptor: 5'-D(*TP*TP*GP*GP*CP*TP*AP*TP*AP*AP*AP*AP*GP*GP*GP*CP*TP*CP*C)-3', 5'-D(*G*GP*AP*GP*CP*CP*CP*TP*TP*TP*TP*AP*TP*AP*GP*CP*CP*AP*A)-3', Transcription Regulator NC2 alpha chain, ... (6 entities in total)
• Functional Keywords: histone, h2a/h2b, tbp, tata-dna, transcription initiation, nc2, negative cofactor, structural genomics, psi, protein structure initiative, new york sgx research center for structural genomics, nysgxrc, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 5
• Total formula weight: 63075.92

Authors

Kamada, K.,Shu, F.,Chen, H.,Malik, S.,Stelzer, G.,Roeder, R.G.,Meisterernst, M.,Burley, S.K.,New York SGX Research Center for Structural Genomics (NYSGXRC) (deposition date: 2001-06-20, release date: 2001-07-11, Last modification date: 2023-08-16)

Primary citation

Kamada, K.,Shu, F.,Chen, H.,Malik, S.,Stelzer, G.,Roeder, R.G.,Meisterernst, M.,Burley, S.K.
Crystal structure of negative cofactor 2 recognizing the TBP-DNA transcription complex.
Cell(Cambridge,Mass.), 106:71-81, 2001

PubMed Abstract: The X-ray structure of a ternary complex of Negative Cofactor 2 (NC2), the TATA box binding protein (TBP), and DNA has been determined at 2.6 A resolution. The N termini of NC2 alpha and beta resemble histones H2A and H2B, respectively, and form a heterodimer that binds to the bent DNA double helix on the underside of the preformed TBP-DNA complex via electrostatic interactions. NC2beta contributes to inhibition of TATA-dependent transcription through interactions of its C-terminal alpha helix with a conserved hydrophobic feature on the upper surface of TBP, which in turn positions the penultimate alpha helix of NC2beta to block recognition of the TBP-DNA complex by transcription factor IIB. Further regulatory implications of the NC2 heterodimer structure are discussed.

PubMed: 11461703
DOI: 10.1016/S0092-8674(01)00417-2

Experimental method

X-RAY DIFFRACTION (2.62 Å)