1JD4

Crystal Structure of DIAP1-BIR2

1JD4 の概要

• エントリーDOI: 10.2210/pdb1jd4/pdb
• 関連するPDBエントリー: 1JD5 1JD6
• 分子名称: APOPTOSIS 1 INHIBITOR, ZINC ION (2 entities in total)
• 機能のキーワード: iap, apoptosis, drosophila, zinc-binding, caspase inhibition
• 由来する生物種: Drosophila melanogaster (fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28288.21

構造登録者

Wu, J.W.,Cocina, A.E.,Chai, J.,Hay, B.A.,Shi, Y. (登録日: 2001-06-12, 公開日: 2001-12-05, 最終更新日: 2023-08-16)

主引用文献

Wu, J.W.,Cocina, A.E.,Chai, J.,Hay, B.A.,Shi, Y.
Structural analysis of a functional DIAP1 fragment bound to grim and hid peptides.
Mol.Cell, 8:95-104, 2001

PubMed Abstract: The inhibitor of apoptosis protein DIAP1 suppresses apoptosis in Drosophila, with the second BIR domain (BIR2) playing an important role. Three proteins, Hid, Grim, and Reaper, promote apoptosis, in part by binding to DIAP1 through their conserved N-terminal sequences. The crystal structures of DIAP1-BIR2 by itself and in complex with the N-terminal peptides from Hid and Grim reveal that these peptides bind a surface groove on DIAP1, with the first four amino acids mimicking the binding of the Smac tetrapeptide to XIAP. The next 3 residues also contribute to binding through hydrophobic interactions. Interestingly, peptide binding induces the formation of an additional alpha helix in DIAP1. Our study reveals the structural conservation and diversity necessary for the binding of IAPs by the Drosophila Hid/Grim/Reaper and the mammalian Smac proteins.

PubMed: 11511363
DOI: 10.1016/S1097-2765(01)00282-9

実験手法

X-RAY DIFFRACTION (2.7 Å)