1JCE

MREB FROM THERMOTOGA MARITIMA

1JCE の概要

• エントリーDOI: 10.2210/pdb1jce/pdb
• 関連するPDBエントリー: 1JCF 1JCG
• 分子名称: ROD SHAPE-DETERMINING PROTEIN MREB (2 entities in total)
• 機能のキーワード: rod-shape determining, mreb, mbl, actin, hsp-70, ftsz, structural protein
• 由来する生物種: Thermotoga maritima
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 37174.74

構造登録者

van den Ent, F.,Amos, L.A.,Lowe, J. (登録日: 2001-06-09, 公開日: 2001-09-19, 最終更新日: 2024-11-13)

主引用文献

van den Ent, F.,Amos, L.A.,Lowe, J.
Prokaryotic origin of the actin cytoskeleton.
Nature, 413:39-44, 2001

PubMed Abstract: It was thought until recently that bacteria lack the actin or tubulin filament networks that organize eukaryotic cytoplasm. However, we show here that the bacterial MreB protein assembles into filaments with a subunit repeat similar to that of F-actin-the physiological polymer of eukaryotic actin. By elucidating the MreB crystal structure we demonstrate that MreB and actin are very similar in three dimensions. Moreover, the crystals contain protofilaments, allowing visualization of actin-like strands at atomic resolution. The structure of the MreB protofilament is in remarkably good agreement with the model for F-actin, showing that the proteins assemble in identical orientations. The actin-like properties of MreB explain the finding that MreB forms large fibrous spirals under the cell membrane of rod-shaped cells, where they are involved in cell-shape determination. Thus, prokaryotes are now known to possess homologues both of tubulin, namely FtsZ, and of actin.

PubMed: 11544518
DOI: 10.1038/35092500

実験手法

X-RAY DIFFRACTION (2.1 Å)