1JBR

Crystal Structure of the Ribotoxin Restrictocin and a 31-mer SRD RNA Inhibitor

1JBR の概要

• エントリーDOI: 10.2210/pdb1jbr/pdb
• 関連するPDBエントリー: 1JBS 1JBT 1aqz 430D 480D 483D
• 分子名称: 5'-R(*GP*CP*GP*CP*UP*CP*CP*UP*CP*AP*GP*UP*AP*CP*GP*AP*GP*(A23))-3', 5'-R(*GP*GP*AP*AP*CP*CP*GP*GP*AP*GP*CP*GP*C)-3', 31-mer SRD RNA analog, ... (6 entities in total)
• 機能のキーワード: protein-rna interaction, specific recognition, restrictocin, ribosomal rna, sarcin/ricin domain, base flipping, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Aspergillus restrictus; 詳細
• 細胞内の位置: Secreted: P67876
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 54046.38

構造登録者

Yang, X.,Gerczei, T.,Glover, L.,Correll, C.C. (登録日: 2001-06-06, 公開日: 2001-10-26, 最終更新日: 2024-10-16)

主引用文献

Yang, X.,Gerczei, T.,Glover, L.T.,Correll, C.C.
Crystal structures of restrictocin-inhibitor complexes with implications for RNA recognition and base flipping.
Nat.Struct.Biol., 8:968-973, 2001

PubMed Abstract: The cytotoxin sarcin disrupts elongation factor binding and protein synthesis by specifically cleaving one phosphodiester bond in ribosomes. To elucidate the molecular basis of toxin action, we determined three cocrystal structures of the sarcin homolog restrictocin bound to different analogs that mimic the target sarcin/ricin loop (SRL) structure of the rat 28S rRNA. In these structures, restrictocin contacts the bulged-G motif and an unfolded form of the tetraloop of the SRL RNA. In one structure, toxin loops guide selection of the target site by contacting the base critical for recognition (G4319) and the surrounding S-shaped backbone. In another structure, base flipping of the tetraloop enables cleavage by placing the target nucleotide in the active site with the nucleophile nearly inline for attack on the scissile bond. These structures provide the first views of how a site-specific protein endonuclease recognizes and cleaves a folded RNA substrate.

PubMed: 11685244
DOI: 10.1038/nsb1101-968

実験手法

X-RAY DIFFRACTION (2.15 Å)