1JAC

A NOVEL MODE OF CARBOHYDRATE RECOGNITION IN JACALIN, A MORACEAE PLANT LECTIN WITH A BETA-PRISM

1JAC の概要

• エントリーDOI: 10.2210/pdb1jac/pdb
• 分子名称: JACALIN, methyl alpha-D-galactopyranoside, ... (4 entities in total)
• 機能のキーワード: non-legume, lectin, beta-prism fold, carbohydrate specificity, glycoprotein
• 由来する生物種: Artocarpus heterophyllus (jackfruit); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 67727.67

構造登録者

Sankaranarayanan, R.,Sekar, K.,Banerjee, R.,Sharma, V.,Surolia, A.,Vijayan, M. (登録日: 1996-05-22, 公開日: 1997-06-05, 最終更新日: 2024-02-07)

主引用文献

Sankaranarayanan, R.,Sekar, K.,Banerjee, R.,Sharma, V.,Surolia, A.,Vijayan, M.
A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold.
Nat.Struct.Biol., 3:596-603, 1996

PubMed Abstract: Jacalin, a tetrameric two-chain lectin (66,000 Mr) from jackfruit seeds, is highly specific for the tumour associated T-antigenic disaccharide. The crystal structure of jacalin with methyl-alpha-D-galactose reveals that each subunit has a three-fold symmetric beta-prism fold made up of three four-stranded beta-sheets. The lectin exhibits a novel carbohydrate-binding site involving the N terminus of the alpha-chain which is generated through a post-translational modification involving proteolysis, the first known instance where such a modification has been used to confer carbohydrate specificity. This new lectin fold may be characteristic of the Moraceae plant family. The structure provides an explanation for the relative affinities of the lectin for galactose derivatives and provides insights into the structural basis of its T-antigen specificity.

PubMed: 8673603
DOI: 10.1038/nsb0796-596

実験手法

X-RAY DIFFRACTION (2.43 Å)