1J59

CATABOLITE GENE ACTIVATOR PROTEIN (CAP)/DNA COMPLEX + ADENOSINE-3',5'-CYCLIC-MONOPHOSPHATE

Replaces:  1BER

Summary for 1J59

• Entry DOI: 10.2210/pdb1j59/pdb
• Descriptor: 5'-D(*GP*CP*GP*AP*AP*AP*AP*GP*TP*GP*TP*GP*AP*C)-3', 5'-D(*AP*TP*AP*TP*GP*TP*CP*AP*CP*AP*CP*TP*TP*TP*TP*CP*G )-3', CATABOLITE GENE ACTIVATOR PROTEIN (CAP), ... (5 entities in total)
• Functional Keywords: protein-dna complex, gene-regulatory, gene regulation-dna complex, gene regulation/dna
• Biological source: Escherichia coli
• Total number of polymer chains: 6
• Total formula weight: 66753.32

Authors

Parkinson, G.,Wilson, C.,Gunasekera, A.,Ebright, Y.W.,Ebright, R.H.,Berman, H.M. (deposition date: 2002-03-01, release date: 2002-03-01, Last modification date: 2023-12-27)

Primary citation

Parkinson, G.,Wilson, C.,Gunasekera, A.,Ebright, Y.W.,Ebright, R.E.,Berman, H.M.
Structure of the CAP-DNA complex at 2.5 angstroms resolution: a complete picture of the protein-DNA interface.
J.Mol.Biol., 260:395-408, 1996

PubMed Abstract: The crystallographic structure of the CAP-DNA complex at 3.0 A resolution has been reported previously. For technical reasons, the reported structure had been determined using a gapped DNA molecule lacking two phosphates important for CAP-DNA interaction. In this work, we report the crystallographic structure of the CAP-DNA complex at 2.5 A resolution using a DNA molecule having all phosphates important for CAP-DNA interaction. The present resolution permits unambiguous identification of amino acid-base and amino acid-phosphate hydrogen bonded contacts in the CAP-DNA complex. In addition, the present resolution permits accurate definition of the kinked DNA conformation in the CAP-DNA complex.

PubMed: 8757802
DOI: 10.1006/jmbi.1996.0409

Experimental method

X-RAY DIFFRACTION (2.5 Å)