1J34

Crystal Structure of Mg(II)-and Ca(II)-bound Gla Domain of Factor IX Complexed with Binding Protein

1J34 の概要

• エントリーDOI: 10.2210/pdb1j34/pdb
• 関連するPDBエントリー: 1BJ3 1IOD 1J35
• 分子名称: coagulation factor IX-binding protein A chain, coagulation factor IX-binding protein B chain, Coagulation factor IX, ... (6 entities in total)
• 機能のキーワード: magnesium ion, calcium ion, gla domain, protein binding-blood clotting complex, protein binding/blood clotting
• 由来する生物種: Trimeresurus flavoviridis; 詳細
• 細胞内の位置: Secreted: P23806 P23807 P00741
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 35641.03

構造登録者

Shikamoto, Y.,Morita, T.,Fujimoto, Z.,Mizuno, H. (登録日: 2003-01-20, 公開日: 2003-07-08, 最終更新日: 2023-11-15)

主引用文献

Shikamoto, Y.,Morita, T.,Fujimoto, Z.,Mizuno, H.
Crystal Structure of Mg2+- and Ca2+-bound Gla Domain of Factor IX Complexed with Binding Protein
J.Biol.Chem., 278:24090-24094, 2003

PubMed Abstract: Factor IX is an indispensable protein required in the blood coagulation cascade. It binds to the surface of phospholipid membrane by means of a gamma-carboxyglutamic acid (Gla) domain situated at the N terminus. Recently, we showed that physiological concentrations of Mg2+ ions affect the native conformation of the Gla domain and in doing so augment the biological activity of factor IXa and binding affinity with its binding protein even in the presence of Ca2+ ions. Here we report on the crystal structures of the Mg2+/Ca2+-bound and Ca2+-bound (Mg2+-free) factor IX Gla domain (IXGD1-46) in complex with its binding protein (IX-bp) at 1.55 and 1.80 A resolutions, respectively. Three Mg2+ and five Ca2+ ions were bound in the Mg2+/Ca2+-bound IXGD1-46, and the Mg2+ ions were replaced by Ca2+ ions in Mg2+-free IXGD1-46. Comparison of Mg2+/Ca2+-bound with Ca2+-bound structures of the complexes showed that Mg2+ ion, which formed a bridge between IXGD1-46 and IX-bp, forced IXGD1-46 to rotate 4 degrees relative to IX-bp and hence might be the cause of a more tight interaction between the molecules than in the case of the Mg2+-free structure. The results clearly suggest that Mg2+ ions are required to maintain native conformation and in vivo function of factor IX Gla domain during blood coagulation.

PubMed: 12695512
DOI: 10.1074/jbc.M300650200

実験手法

X-RAY DIFFRACTION (1.55 Å)