1IXR

RuvA-RuvB complex

1IXR の概要

• エントリーDOI: 10.2210/pdb1ixr/pdb
• 関連するPDBエントリー: 1HQC 1IXS
• 分子名称: Holliday junction DNA helicase ruvA, RuvB, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER (3 entities in total)
• 機能のキーワード: heterooligomeric complex, octameric ruva, aaa-atpase domain, complex with nucleotide, hydrolase
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 76189.62

構造登録者

Yamada, K.,Miyata, T.,Tsuchiya, D.,Oyama, T.,Fujiwara, Y.,Ohnishi, T.,Iwasaki, H.,Shinagawa, H.,Ariyoshi, M.,Mayanagi, K.,Morikawa, K. (登録日: 2002-07-04, 公開日: 2002-11-06, 最終更新日: 2023-12-27)

主引用文献

Yamada, K.,Miyata, T.,Tsuchiya, D.,Oyama, T.,Fujiwara, Y.,Ohnishi, T.,Iwasaki, H.,Shinagawa, H.,Ariyoshi, M.,Mayanagi, K.,Morikawa, K.
Crystal Structure of the RuvA-RuvB Complex: A Structural Basis for the Holliday Junction Migrating Motor Machinery
Mol.Cell, 10:671-681, 2002

PubMed Abstract: We present the X-ray structure of the RuvA-RuvB complex, which plays a crucial role in ATP-dependent branch migration. Two RuvA tetramers form the symmetric and closed octameric shell, where four RuvA domain IIIs spring out in the two opposite directions to be individually caught by a single RuvB. The binding of domain III deforms the protruding beta hairpin in the N-terminal domain of RuvB and thereby appears to induce a functional and less symmetric RuvB hexameric ring. The model of the RuvA-RuvB junction DNA ternary complex, constructed by fitting the X-ray structure into the averaged electron microscopic images of the RuvA-RuvB junction, appears to be more compatible with the branch migration mode of a fixed RuvA-RuvB interaction than with a rotational interaction mode.

PubMed: 12408833
DOI: 10.1016/S1097-2765(02)00641-X

実験手法

X-RAY DIFFRACTION (3.3 Å)