1IUB

Fucose-specific lectin from Aleuria aurantia (Hg-derivative form)

1IUB の概要

• エントリーDOI: 10.2210/pdb1iub/pdb
• 関連するPDBエントリー: 1IUC
• 分子名称: Fucose-specific lectin, beta-L-fucopyranose, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: hg, mad, lectin, riken structural genomics/proteomics initiative, rsgi, structural genomics, sugar binding protein
• 由来する生物種: Aleuria aurantia (orange peel mushroom)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34322.44

構造登録者

Fujihashi, M.,Peapus, D.H.,Kamiya, N.,Nagata, Y.,Miki, K.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2002-03-01, 公開日: 2003-09-30, 最終更新日: 2023-12-27)

主引用文献

Fujihashi, M.,Peapus, D.H.,Kamiya, N.,Nagata, Y.,Miki, K.
Crystal Structure of Fucose-Specific Lectin from Aleuria aurantia Binding Ligands at Three of Its Five Sugar Recognition Sites
Biochemistry, 42:11093-11099, 2003

PubMed Abstract: Aleuria aurantia possesses a fucose-specific lectin (AAL) that is widely used as a specific probe for fucose. Fucosylated sugars often play pivotal roles in many cellular processes. We have determined the crystal structure of AAL at 2.24 A resolution in complex with only three fucose molecules in its five sugar binding sites of a six-fold beta-propeller structure. Very recently, the structure of AAL has been independently determined, showing that all the five binding sites were occupied by fucose molecules [Wimmerova, M., et al. (2003) J. Biol. Chem. 278, 27059-27067]. Stabilization of the arginine conformation bound to fucose molecules plays an essential role in generating the difference in the affinity in the five binding sites. Binding models with a couple of saccharides based on biochemical assays suggest that hydrophobic contacts also play important roles in AAL recognizing its ligand.

PubMed: 14503859
DOI: 10.1021/bi034983z

実験手法

X-RAY DIFFRACTION (2.31 Å)