1ISK

3-OXO-DELTA5-STEROID ISOMERASE, NMR, 20 STRUCTURES

1ISK の概要

• エントリーDOI: 10.2210/pdb1isk/pdb
• 分子名称: 3-OXO-DELTA5-STEROID ISOMERASE (1 entity in total)
• 機能のキーワード: isomerase, ksi, 3-ketosteroid
• 由来する生物種: Comamonas testosteroni
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 26824.20

構造登録者

Wu, Z.R.,Ebrahimian, S.,Zawrotny, M.E.,Thornburg, L.D.,Perez-Alvarado, G.C.,Brothers, P.,Pollack, R.M.,Summers, M.F. (登録日: 1997-03-12, 公開日: 1997-11-12, 最終更新日: 2024-05-22)

主引用文献

Wu, Z.R.,Ebrahimian, S.,Zawrotny, M.E.,Thornburg, L.D.,Perez-Alvarado, G.C.,Brothers, P.,Pollack, R.M.,Summers, M.F.
Solution structure of 3-oxo-delta5-steroid isomerase.
Science, 276:415-418, 1997

PubMed Abstract: The three-dimensional structure of the enzyme 3-oxo-delta5-steroid isomerase (E.C. 5.3.3.1), a 28-kilodalton symmetrical dimer, was solved by multidimensional heteronuclear magnetic resonance spectroscopy. The two independently folded monomers pack together by means of extensive hydrophobic and electrostatic interactions. Each monomer comprises three alpha helices and a six-strand mixed beta-pleated sheet arranged to form a deep hydrophobic cavity. Catalytically important residues Tyr14 (general acid) and Asp38 (general base) are located near the bottom of the cavity and positioned as expected from mechanistic hypotheses. An unexpected acid group (Asp99) is also located in the active site adjacent to Tyr14, and kinetic and binding studies of the Asp99 to Ala mutant demonstrate that Asp99 contributes to catalysis by stabilizing the intermediate.

PubMed: 9103200
DOI: 10.1126/science.276.5311.415

実験手法

SOLUTION NMR